By Walker J.M. (ed.), Pollard J.W. (ed.)
CIP cites the sequence because the major access. Covers tools for developing mammalian fibroblastic phone cultures and protecting tradition stipulations for epithelial, neuronal, and hematopoietic cells between others. recognition is given to the range of tradition media and extracellular matrices had to keep the differential capabilities of the aesthetic cells. additionally describes tradition ideas for either residing and glued cells.
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Additional info for Animal Cell Culture
Hollow fiber). A disadvantage is the high substrate cost $1800-225O/kg (E120041500/kg) and the fastidious nature of the technique. References I. 2. 3. 4. 5. Spier, R. E. and Griffiths, J. B. (1983) An examination of the data and concepts germane to the oxygenation of cultured animal cells. Develop0Bid. 55,81-92. B. ), IRL Press,Oxford, pp. 33-70. Griffiths, J. , Cameron, D. , and Looby, D. (1987) A comparison of unit process systems for anchorage dependent cells. Develop. Bid. Sfunduyd,66,331-338.
The technique can be amended if particular problems are anticipated with cells. SeeNotes 4-7. Lyrnphoblastoid Cell Lines 4. Notes 1. A major consideration is the source of EBV. 8 (4) as a source of virus, although in some cases cocultivation with the cell line QIMR-WIL has been employed. 8 cell lines distributed informally between research laboratories are mycoplasma contaminated. It is therefore of the utmost importance that the basic starting material for the technique, the virus source, is obtained from a reliable source, such as an established culture collection.
2. Water jacket temperature control to avoid the use of immersion heaters that give localized high temperatures. Electrically operated silicone pads are also suitable at volumes up to 5 L-the only disadvantage is the reduction in visibility into the vessel. 3. Absence of baffles and other sharp protrusions that cause turbulence. The interior is finished to a high grade of smoothness to minimize mechanical damage and for cleanliness. 4. An aspect ratio (height to diameter) of 2:l maximum, and preferably no more than X5:1.
Animal Cell Culture by Walker J.M. (ed.), Pollard J.W. (ed.)